Silver nanoclusters as label free non-enzymatic fast glucose assay with the fluorescent enhancement signal

Abstract

Abstract A simple and low-cost green preparation method was used for BSA capped silver nanoclusters (BSA-Ag NCs) as turn on fluorescent probe for glucose. Non-enzymatic fast glucose detection assay with a widest concentration range was proposed which requires neither nanoclusters (NCs) modification nor complicated enzyme immobilization. The DLS analysis, HRTEM patterns, fluorescence and UV-visible measurement well supported the synthesis product. The advantages of the fabricated glucose sensor based on fluorescence increasing of probe compared to other established optical techniques was inspected and summarized as well. The glucose sensor exhibited a high sensitivity, fast response time (in seconds), satisfactory selectivity, well stability (at least two months), low detection limit (31 µmol L− 1) and a wide concentration response (three orders of magnitudes) to glucose between 0.1 to 92 mmol L− 1 as calibration plot. The facile preparation and excellent sensing performance of BSA-Ag NCs in the real samples (plasma and juice) make sure that synthesized probe material is a promising candidate for advanced enzyme-free glucose sensing approach.