Affiliation:
1. Universidade de Uberaba (UNIUBE)
2. Federal University of Uberlândia
Abstract
Abstract
Background:
This study aimed to evaluate the efficiency of stromal derived from adipose tissue in the treatment of osteochondral defects in rabbits. A total of 36 white New Zealand rabbits were used: 24 to perform an osteochondral defect in the trochlear groove of the joints and 12 to collect adipose tissue in the region of the adipose panicle, divided in three digestion times (30, 45 and 50 minutes). The groups were divided into negative control (C-), stromal fraction of adipose tissue (SFAT) and positive control (C+) with autogenous osteochondral graft.
Results:
Clinical, radiographic, macroscopic and histopathological evaluations showed that the therapeutic effects of the stromal fraction of adipose tissue minimized the degenerative signs of articular cartilage in the treatment of osteochondral defects in rabbits.
Conclusion:
The digestion time of 30 minutes was the best option for obtaining the stromal fraction from adipose tissue of rabbits and its use at the lesion site was satisfactory, without any immunological complications, indicating potential as an alternative for the treatment of osteochondral defects.
Publisher
Research Square Platform LLC
Reference24 articles.
1. Papel da via de sinalizacão do HIF-1 alfa na osteoartrite: revisão sistemática;Torres JF;Rev Bras Reumatol,2017
2. Clinical and MRI evaluation of420 medium- to long-term results after autologous osteochondral transplantation (OCT) in the knee joint;Zak L;Knee Surg Sports Traumatol Arthrosc,2014
3. Stefanutto JC, Dal Molin MM, Emanuelli IP. Técnicas Para Isolamento de Células Tronco Mesenquimais Extraídas de Tecido Adiposo. Presented at: VIII EPCC – Encontro Internacional de Produção Científica UniCesumar, Octuber 25, 2013; Maringá, PR. http://www.cesumar.br/prppge/pesquisa/epcc2013/oit_mostra/Jessica_Cristina_Stefanutto_02.pdf Accessed 01 Jun 2023.
4. Concise Review: Stem Cell Trials Using Companion Animal Disease Models;Hoffman AM;Stem Cells,2016
5. Mesenchymal Stem Cells - Emphasis in Adipose Tissue;Bertassoli BM;Braz Arch Biol Technol,2013