The effect of methanolic extract of Spirogyra varians on biofilm genes expression of Staphylococcus aureus

Abstract

Abstract Background and goals: Methicillin resistance Staphylococcus aureus (MRSA) is one of the most significant antibiotic resistant bacteria, that can produce biofilm. Which has seriously hampered patient care, Twenty to thirty percent of people carry this bacteria with no clinical symptoms appear. New strategies were required to eradicate the effect of this bacteria on health care, so this study aimed to evaluate biofilms gene expression levels in MRSA after treated with alga Spirogyra extract. Materials and Methods Soxhlet extract of the Spirogyra varians species was made in methanol, the minimum inhibitory concentration (MIC) of methanol extract of Spirogyra toward S. aureus was detect. PCR technique was used to Real Time PCR was used to measure the desired genes' expression levels at doses below the minimum inhibitory concentration (MIC). Results the Spirogyra extract MIC toward S. aureus was 200 mg/ml, the effect of extract on genes the significantly changed the expression of genes involved in MRSA biofilm formation. After being treated with algal extracts from Spirogyra, the expression level of icaA and icaD were 0.480, 0.688, respectively, icaD expression level was correspondingly high. Conclusion This study showed that Spirogyra methanol extract can prevent S. aureus from forming biofilms.