Hsa_circ_0008344 promotes glioma cells proliferation, invasion and migration via miR-556- 5p/NLRP3/NF-κB signaling pathway

Abstract

Abstract The circular RNAs (circRNA) are becoming more important in cancer development. However, the functional role of circRNA in glioblastoma is still remain to be deeply studied. In the current study, we used CCK-8, flow cytometry, transwell, western blot, and RT-PCR assays to determine the expression of has_circ_0008344 in different glioma cells. Results showed that the expression level of hsa_circ_0008344 in glioma cells was remarkably higher than normal HCGC cells. Down-regulation of hsa_circ_0008344 significantly decreased the proliferation rate, increased apoptotic rate, impaired invasion and migration capacity in U87MG and U251MGA cells. We found that miR-513a-5p mimics could significantly reverse the over-expression of hsa_circ_0008344 and enhancing the knocking-down of hsa_circ_0008344. Moreover, NLRP3 promoted the biological activities of glioma cells coordinately with hsa_circ_0008344 and could reverse the impact of miR-513a-5p mimics on over-expression of hsa_circ_0008344 treated glioma cells. Also activated p-NF-κB involved in the invasion and migration of glioma cells. In conclusion, the present study demonstrates that hsa_circ_0008344 is up-regulated in glioma in vitro and promoted the proliferation activity, invasion and migration of glioma cells by ameliorating miR-556-5p/NLRP3/NF-κB signaling pathway. Our results may contribute to the diagnosis and treatment of glioma in the future.