Polymorphism of FEN1 Gene is Associated with DNA Damage of Cytb Gene in Arsenic-Exposed Workers

Abstract

Abstract Objective: To explore the relationship between Polymorphism of FEN1 Gene and DNA Damage of Cytb Gene in Arsenic-Exposed Workers. Methods: The content of arsenide in urine of 79 arsenic-exposed workers and 24 agricultural workers was detected by an atomic absorption spectrophotometer. The DNA damage level of Cytb gene and the polymorphism of FEN1 gene in peripheral blood were detected by polymerase chain reaction. Results: The increased exposure to arsenic caused DNA damage of Cytb gene, resulting in an increase in the content of arsenide in urine and hypomethylation of arsenic metabolism. Meanwhile, there was a potential positive correlation between the FEN1 rs174538 AA or GA+AA allele and the DNA damage level of Cytb gene. Conclusions: The DNA damage of Cytb gene caused by arsenic exposure is related to FEN1 gene polymorphism, the content of arsenide in urine and the level of arsenic methylation metabolism.