Genome interrogation of homeostatic calcium activity using CaMP-screen-Reference-Cited by-同舟云学术

Genome interrogation of homeostatic calcium activity using CaMP-screen

Published:2024-07-22 Issue: Volume: Page:
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Author:

Carreras-sureda Amado¹,Kouba Sana²,Zhang Xin²,Castelbou Cyril²,Demaurex Nicolas³^ORCID

Affiliation:

1. Department of Cell Physiology and Metabolism, University of Geneva

2. University of Geneva, Department of Cell Physiology and Metabolism

3. University of Geneva

Abstract

Calcium signals regulate crucial cellular functions yet many genes coding for Ca²⁺handling proteins remain unknown as their identification relies on low-throughput single-cell approaches. Here we describe a novel method to measure Ca²⁺ activity in cells isolated by flow cytometry following pooled genome interrogation. Using a CRISPR/ CAMPARI2 screen, we identified enhancers and inhibitors of homeostatic Ca²⁺ activity.

Publisher

Springer Science and Business Media LLC

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