Stereocomplexed microparticles loaded with Salvia cadmica Bioss. extracts for enhancement of immune response towards Helicobacter pylori

Abstract

Abstract Introduction Controlled delivery of therapeutic substance gives numerous advantages (prevents degradation, improves uptake, sustains concentration, lowers side effects). Aim To encapsulate Salviae cadmica extracts (root or aerial part), enriched with polyphenols with immunomodulatory activity, in stereocomplexed microparticles (sc-PLA), resistant to the acidic and basic pH, for using them to enhance the immune response towards gastric pathogen Helicobacter pylori. Material and methods Microparticles were made of biodegradable poly(lactic acid) (PLA) and poly(lactic-co-glycolic acid) (PLGA). Stereocomplexation method was used to improve scPLA stability in acidic/basic pH, and to release extracts in pH 8.0, which is in the gut being the center of mucosal immunity. Results The obtained polymers are safe in vitro and in vivo (guinea pig model). The sc-PLA modifications enable the release of S. cadmica extracts in pH 8.0. S. cadmica extracts enhanced the phagocytic activity of guinea pig bone marrow derived macrophages, which was diminished by H. pylori, and neutralized H. pylori driven enhanced production of tumor necrosis factor (TNF)-α and interleukin (IL)-10. Concusion The sc-PLA encapsulated S. cadmica extracts can be recommended for further in vivo study in guinea pigs infected with H. pylori to confirm their ability to improve an immune response towards this pathogen.