Predictive effect and clinical significance of exosome-related genes for Nonalcoholic fatty liver disease-related hepatocellular carcinoma-Reference-Cited by-同舟云学术

Predictive effect and clinical significance of exosome-related genes for Nonalcoholic fatty liver disease-related hepatocellular carcinoma

Published:2023-04-03 Issue: Volume: Page:
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Author:

Ding Xiaoqian¹,Zhang Jie¹,Ma Xuefeng¹,Sun Baokai¹,Chu Xueru²,Liu Shousheng³,Zhao Zhenzhen³,Chen Lizhen¹,Xin Yongning¹,Zhuang Likun³

Affiliation:

1. Department of Infectious Diseases, Qingdao Municipal Hospital, Qingdao University

2. School of Medicine and Pharmacy, Ocean University of China

3. Clinical Research Center, Qingdao Municipal Hospital, Qingdao University

Abstract

AbstractNonalcoholic fatty liver disease (NAFLD) is a multifactorial metabolic syndrome which could develop into hepatocellular carcinoma (HCC). Exosomes could mediate intercellular communications and play key roles in the occurrence and development of many diseases including NAFLD and HCC. However, expressions and roles of the genes related to exosomes in NAFLD-related HCC remain unclear. The objective of this study was to investigate the expressions and diagnostic values of exosome-related genes in NAFLD-related HCC. Firstly, we identified 15 genes related to exosome secretion by database searching. Two subgroups of patients with NAFLD-related HCC from GEO database were identified on the basis of exosome-related genes. High level of memory CD4 + T cells and low expressions of Immune Checkpoint genes were observed in the group with highly expressed genes which could promote exosome secretion. Subsequently, the logistic regression analysis was used to generate the formula based on three selected exosome-related genes in NAFLD-related HCC cohort. Receiver operating characteristic (ROC) curve analysis confirmed the good predictive power of formula. Functional analysis showed abundant metabolism-related pathways were screened out in DEGs between the high and low risk-score groups. The risk score from the formula was also positively correlated with memory CD4 + T cells and negatively associated with Immune Checkpoint genes. Finally, prognostic potential of the Exosome-related formula was analyzed in HCC cohort from TCGA database, and patients in the group with highly expressed exosome-promoted genes showed significantly reduced overall survival. We also constructed a nomogram to improve the predictive value of the formula for evaluating the prognosis of patients with HCC. In summary, our study clarified the significance of exosome-related genes in the development of NAFLD-related HCC.

Publisher

Research Square Platform LLC

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