The impact of intermittent and continuous training on the levels of CIDE and Perilipin-1 proteins and their effect on the size of lipid droplets in the visceral adipose tissue of obese male rats

Author:

Bai Yan1,Yang Xiao1,Li Jian2

Affiliation:

1. Guangzhou Civil Aviation College

2. Guangzhou Sport University

Abstract

Abstract

Background: Intense interval training and moderate-intensity continuous exercise produce lipid droplets that change size and impact visceral adipose tissue. Methods: 50 male Wistar rats were divided into 5 groups, each consisting of 8 rats, in order to accomplish this objective. Regarding dietary intake, 2 clusters of 32 rats were subjected to a normal or elevated fat diet over 10 weeks. Post the induction of obesity, 16 animals were euthanized, with an equal number originating from both the high-fat and normal diet cohorts. The ramifications of a high-fat diet were examined through the utilization of samples. The remaining 24 rats were randomly allocated to 3 groups: a sedentary high-fat diet control group, a high-intensity interval training (HIIT) protocol group, and a moderate-intensity continuous training (MICT) protocol group. The 12-week training program had 5 sessions per week. Western blot measurement of perilipin-1, CIDE, and Oil-Red proteins assessed lipid droplet size. Results:Research indicates that HIIT and MICT training significantly decreased CIDEc protein levels (p<0.05) but not CIDEa. CIDEc protein upregulation and perilipin-1 downregulation cause obesity in high-fat diets. Conclusion:HIIT and MICT training reduce fat droplet size and CIDEc protein production. Enhancing perilipin-1, which breaks down fats, may reduce obesity by lowering lipid droplets and weight.

Publisher

Research Square Platform LLC

Reference27 articles.

1. Packaging of fat: An evolving model of lipid droplet assembly and expansion;Brasaemle DL;Journal of Biological Chemistry,2012

2. Lipid droplet-organelle interactions; sharing the fats. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology;Murphy S;of Lipids,2009

3. Lipid droplet growth: Regulation of a dynamic organelle;Barneda D;Current Opinion in Cell Biology,2017

4. Quantitative analysis of lipid droplet fusion: Inefficient steady state fusion but rapid stimulation by chemical fusogens;Murphy S;PloS one,2010

5. Control of lipid droplet fusion and growth by cide family proteins. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology;Gao G;of Lipids,2017

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3