Kaposi’s sarcoma-associated herpes virus (KSHV) do not imply the vast majority of Sri Lankan oral cancer patients

Abstract

Abstract Human Herpes virus 8 (HHV 8) better known as Kaposi’s sarcoma-associated herpes virus (KSHV) frequently relates to malignancies in acquired immune deficiency syndrome (AIDS) patients. Interestingly, one incident reported in a head and neck cancer patient who is also a HIV patient. In the light of these findings, this original research aimed to detect the HHV-8 status in oral squamous cell carcinoma tissues of Sri Lankan male patients. Incisional biopsies of cases were collected, transported, stored, and dispatched as frozen tissues at -800 C. A sub case sample selected from a large case control study to represent the vast majority of the oral cancer patients in Sri Lankan context as oral cancer remains number 1 among Sri Lankan males. The DNA was extracted from frozen specimens using Gentra Puregene Tissue kit (Qiagen, Germany), solid tissue protocol according to the manufacturer’s instructions. The real-time PCR was performed with the specific primers to find out the possible implication of this virus on the vast majority of Sri Lankan oral cancer patients with established socio-demographic and risk factor profiles. Data belonged to Sociodemographic, oral hygiene practices, oral hygiene status, clinical and oral risk habit categories were obtained by a pre tested interviewer administered questioner were entered and analyzed using SPSS-21 Statistical Package. However, the HHV-8 DNA did not find in 22 OSCC cases. Kaposi’s sarcoma-associated herpes virus (KSHV) does not imply the vast majority of Sri Lankan oral cancer patients. Powered studies on minority [the high risk human papilloma virus (HPV) associated] oral cancer patients are recommended as transmission of both HPV and KSHV seems same among homosexuals.