In vitro naive CD4+ T cells differentiation upon treatment with miR-29b-loaded exosomes from Mesenchymal Stem Cells

Abstract

Abstract Background: Gene regulation by microRNA is central in T cell differentiation processes. Here, we investigate miR-29b roles in the reprogramming of T cell differentiation, which can be a promising therapeutic avenue for various types of inflammatory disorders such as rheumatoid arthritis and multiple sclerosis. Methods and Results: Adipose Mesenchymal Stem Cell derived exosomes (AMSC-Exo) enriched with miR-29b were delivered into naive CD4+ T cells (nCD4+) cells. The expression level of some important transcription factors including RORγt, GATA3, T-bet, and Foxp3 was determined by quantitative Real-Time PCR (qPCR). Moreover, flow cytometry and Enzyme-linked Immunosorbent Assay (ELISA) was respectively used to measure the frequency of T regulatory cells and the levels of cytokines production (IL-17, IL-4, IFN-γ, and TGF-β). This study indicates that the transfection of miR-29b mimics into T lymphocytes through AMSC-Exo can alter the CD4+ T cells differentiation into other types of T cells. Conclusions: In conclusion, AMSC-Exo-based delivery of miR-29b can be considered as a new fascinating avenue for T cell differentiation inhibition and the future treatment of several inflammatory disorders.