Affiliation:
1. Department of Bionanoscience, Delft University of Technology, Delft, Netherlands
Abstract
Abstract
tRNAs in bacteriophage genomes are widespread across bacterial genera, but their exact function has remained unclear for more than 50 years. Multiple hypotheses have been proposed, with the most established being codon compensation, where codons more rarely used by the host but necessary for the phage are supplemented by tRNAs encoded by the phage. Here, we combine several observations and propose a new hypothesis that phage-encoded tRNAs are a means to counteract the tRNA-depleting strategies of the host to defend from viral infection. Based on mutational patterns of tRNA anticodon loops, we predict that phage tRNAs are insensitive to the host tRNAses. For tRNAs targeted in the anticodon itself, we observe phage counter-selection of targeted isoacceptor tRNAs, further supporting the hypothesis that phage tRNAs are selected to be insensitive to host anticodon nucleases. Importance The presence of tRNAs in phages was discovered more than 50 years ago and their function has been debated ever since. Here, we propose that phage tRNAs counteract the tRNAse activities of the host, which may represent a depletion strategy of essential cellular components to stop translation and thereby phage infection.
Funder
European Research Council
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Publisher
Research Square Platform LLC
Reference25 articles.
1. In vitro reconstitution of anticodon nuclease from components encoded by phage T4 and Escherichia coli CTr5X;Amitsur M;The EMBO Journal,1989
2. Causes for the intriguing presence of tRNAs in phages;Bailly-Bechet M;Genome Research,2007
3. Barth, V. C., Chauhan, U., Zeng, J., Su, X., Zheng, H., Husson, R. N., & Woychik, N. A. (2021). Mycobacterium tuberculosis VapC4 toxin engages small ORFs to initiate an integrated oxidative and copper stress response. Proceedings of the National Academy of Sciences, 118(32), e2022136118.
4. Apoptosis like symptoms associated with abortive infection of Mycobacterium smegmatis by mycobacteriophage D29;Calcuttawala F;Plos one,2022
5. Interaction of colicin E4 with specific receptor sites mediates its cleavage into two fragments inactive towards whole cells;Cavard D;European Journal of Biochemistry,1979
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