Transcriptome analysis and identification of the low potassium stress-responsive gene SiSnRK2.6 in foxtail millet (Setaria italica L.)

Abstract

Abstract Foxtail millet (Setaria italica L.), which originated in China, has typical drought tolerance, barren tolerance, C4 high light efficiency, strong adaptability, and high nutrient utilization characteristics. Nevertheless, the molecular mechanism of its tolerance to low-potassium stress is largely unclear. In this research, the low potassium tolerant variety “Yugu28” was screened out by low potassium stress treatment, and the transcriptome of “Yugu28” under low potassium was comprehensively analyzed. A total of 4254 differentially expressed genes (DEGs) were identified, including 1618 up-regulated and 2636 down-regulated genes, respectively. In addition, there were 302 transcription factor (TF) genes in the DEGs and MYB TFs accounted for the highest proportion, which was 14.9%. After functional analysis of all DEGs, a total of 7 genes directly related to potassium and 50 genes corresponding to hormones were screened. The expression levels of randomly selected 17 DEGs were verified by qRT-PCR and the results coincided well with the RNA-seq analysis, indicating the reliability of our transcriptome data. Moreover, one of the ABA signaling pathway genes, SiSnRK2.6, was identified and selected for further functional verification. Compared with the wild-type, transgenic rice with ecotopic expression of SiSnRK2.6 showed remarkably increased root length and root number, indicating that overexpression of SiSnRK2.6 can enhance the resistance of transgenic plants to low potassium stress.