Abstract
Phenotypic changes in energy metabolism are one of the key reasons for changes in brain function during the development of neurodegenerative diseases. The aim of the work was to study cell respiration features and the energy phenotype in inbred mouse strains. The primary mixed culture of hippocampal neurons isolated from embryos on the 18th day of gestation and from newborn mice on the 2nd day was used. Based on the measurement results maps of the metabolic profile of the neuron-glia culture was constructed. As a result, of the study, an increased oxygen consumption rate was established both in the E18 culture by 16.95 (p < 0.05) and in P2 by 32% (p < 0.05) in APP mice. The lowest level of basal mitochondrial respiration was characteristic of the primary mixed culture of neurons obtained from HPRT mice in the E18 culture, the oxygen consumption rate decreased by 23.7% (p < 0.05), in the P2 culture - by 52.4% (p < 0.05). On the E18 and P2 hippocampal culture derived from mice with models of neurodegeneration (APP, Tau) revealed an increased bioenergetics demand, an aerobic type of metabolism and the absence of switching the energy phenotype under physiological conditions were established. The identified features should be taken into account in the further when planning experiments to study the mechanisms of influence of various pharmacological substances on the respiratory function of neurons.