Complete genome sequence of a novel dsRNA virus from the phytopathogenic fungus Fusarium oxysporum

Abstract

Abstract Fusarium oxysporum is a widespread plant pathogen that causes Fusarium wilt and Fusarium root rot in many economically significant crops. Here, a novel dsRNA virus tentatively named Fusarium oxysporum Virus 1 (FoV1) was identified from F. oxysporum strain 3S-18. The genome of FoV1 spans a length of 2,944 nucleotides (nt) and encompasses two distinct open reading frames (ORF1-2), which do not overlap with each other. The larger ORF2 encodes RNA-dependent RNA polymerases (RdRp) of 590 amino acids with a molecular mass of 67.52 kDa. The ORF1 encodes a putative nucleocapsid protein consisting of 134 amino acids with a molecular mass of 34.25 kDa. The RdRp domain of FoV1 shares 60.00–84.24% sequence similarity to the non-segmented dsRNA virus. Phylogenetic analysis further suggested that FoV1 is a new member of the unclassified monopartite dsRNA virus.