Affiliation:
1. National Institute of Technology
2. University of Notre Dame
Abstract
Abstract
Along with health concern, choosing of plants as bioactive phytochemical source to synthesize nanoparticles is highly demanding due to the biocompatibility, nontoxicity, and cost-effectiveness over other available physical and chemical methods. Here, for the first time, Coffee arabica leaf extracts (CAE) were used to produce highly stable silver nanoparticles (AgNPs) and corresponding bio reduction, capping and stabilization mechanism mediated by 5-caffeoylquinic acid (5-CQA) is discussed. UV-Vis, FTIR, Raman spectroscopy, TEM, DLS and Zeta potential analyzer measurements were used to characterize these green synthesized NPs. The affinity of 5-CQA capped CAE-AgNPs to thiol moiety of amino acid is utilized for the selective as well as sensitive detection of L-cysteine (Cys) to a low detection limit of 0.1nM. Hence, the proposed novel, simple, eco-friendly, and economically sustainable method can provide a promising nanoplatform in the field of biosensors which is compliant with large-scale industrial production of AgNPs without aid of instruments.
Publisher
Research Square Platform LLC
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