Longitudinal survey of insecticide resistance in a village of Central Region of Burkina Faso reveals co-occurrence of 1014F, 1014S and 402L mutations in Anopheles coluzzii and Anopheles arabiensis

Abstract

Introduction. Pyrethroid resistance is one of the major threats for effectiveness of insecticide-treated bed nets (ITNs) in malaria vector control. Genotyping of mutations in the voltage gated sodium channel (VGSC) gene is widely used to easily assess the evolution and spread of pyrethroid target-site resistance among malaria vectors. L1014F and L1014S substitutions are the most common and best characterized VGSC mutations in major African malaria vector species of the Anopheles gambiae complex. Recently, an additional substitution involved in pyrethroid resistance i.e. V402L, has been detected in Anopheles coluzzii from West Africa lacking any other resistance alleles at locus 1014. We here monitored the evolution of target-site resistance mutations L1014F/S and V402L in A. coluzzii and A. arabiensis specimens from a Burkina Faso village over a 10-year range after the massive ITN scale-up started in 2010. Methods. A. coluzzii (N = 300) and A. arabiensis (N = 362) specimens collected in 2011, 2015 and 2020 at Goden village were genotyped by TaqMan assays and sequencing for the three target site resistance mutations; allele frequencies were statistically investigated over the years. Results. A divergent trend in resistant allele frequencies was observed in the two species: 1014F decreased in A. coluzzii (from 0.76 to 0.52) but increased in A. arabiensis (from 0.18 to 0.70); 1014S occurred only in A. arabiensis and slightly decreased over time (from 0.33 to 0.23); 402L increased in A. coluzzii (from 0.15 to 0.48) and was found for the first time in one A. arabiensis specimen. In 2020 the co-occurrence of different resistance alleles reached 43% in A. coluzzii (alleles 410L and 1014F) and 32% in A. arabiensis (alleles 1014F and 1014S). Conclusions. Overall, an increasing level of target-site resistance was observed among the populations with only 1% of the two malaria vector species being wild type at both loci, 1014 and 402, in 2020. This, together with the co-occurrence of different mutations in the same specimens, calls for future investigations on the possible synergism between resistance alleles and their phenotype to implement local tailored intervention strategies.