Bioinformatics analysis of IGFBP-3 and research advances in brain tumors Running title：Protein structure and biological function

Abstract

Abstract OBJECTIVE：Bioinformatics analysis of human insulin-like growth factor-binding protein-3 (IGFBP-3) molecules and genes, and review of the pathways and regulatory mechanisms of IGFBP-3 in brain tumors ( especially gliomas ) . METHODS：Analysis of IGFBP-3 protein open reading frame（ORF）and physicochemical properties, secondary structure, tertiary structure, signal peptide and nuclear localization signal, transmembrane structure, domain, phosphorylation and glycosylation of IGFBP-3 protein using molecular biology tools Sites, proteins and biological functions that interact with IGFBP-3 proteins. RESULTS：The molecular formula of IGFBP-3 protein is C1390H2230N418O422S21. 104 ORFs were found in the mRNA sequence of IGFBP-3. The longest ORF is ORF2, which is 519 bp in total and can encode 172 amino acids.It is an unstable hydrophilic secreted protein, the secondary structure is mainly ring structure and helical structure, containing 67 phosphorylation sites and 3 N-glycosylation sites, no transmembrane region, 3 main domains, Having nuclear localization signals exerts biological effects in the nucleus. IGFBP-3 can participate in biological functions such as apoptosis, negative regulation of cell proliferation, negative regulation of signal transduction, and regulation of insulin-like growth factor receptor signaling pathway. CONCLUSIONS：The predicted results provide a basis for further research on the structure and biological function of IGFBP-3 gene and its encoded protein.