Can Wastewater-based Surveillance Relate Antimicrobial Use to Resistance Patterns? Metagenomic Analysis of Two Neighboring Treatment Plants

Author:

Vikesland Peter1ORCID,Maile-Moskowitz Ayella1ORCID,Brown Connor1,Rumi Monjura1,Moumi Nazifa1,Majeed Haniyyah1,Finkielstein Carla1,ceci alessandro2ORCID,Gonzalez Raul3,Xia Kang1,McDaniel Lauren4,Baffoe-Bonnie Anthony4,Rao Jayasimha4,Zhang Liqing1,Pruden Amy1

Affiliation:

1. Virginia Tech

2. Molecular Diagnostics Laboratory, Fralin Biomedical Research Institute, Virginia Tech

3. Hampton Roads Sanitation District

4. Carilion Clinic

Abstract

Abstract

Minimization of the use of antimicrobials is a recommended strategy to reduce the evolution and spread of antibiotic resistance; however, challenges remain in assessing the efficacy of such interventions. Wastewater-based surveillance (WBS) provides a promising means to consider differences in antibiotic resistance profiles across communities as a function of such interventions. We examined the metagenomes of sewage entering two neighboring wastewater treatment plants (WWTPs) serving a university and a nearby community. We compared antibiotic resistance gene (ARG) profiles as a function of data available for diagnoses of COVID-19 and other illnesses, clinical antibiotic use, antibiotic/antimicrobial and disinfectant/quaternary ammonium compound (QAC) concentrations in sewage, water quality indicators, and COVID-19-related behavioral shifts. Outpatient antibiotic prescription rates were consistently higher in the community than in the university zip code, but dropped markedly in both towns in April 2020, as COVID-19 lockdowns ensued. Antibiotic and QAC concentrations in sewage were consistent with these trends. Diversity and abundances of ARGs unique to the corresponding sewage were consistently higher for the community WWTP, but converged in 2022 when there was a surge in antibiotic prescriptions in the university zip code in response to elevated respiratory illness. Decreases in ARG diversity/abundance were not apparent during periods of decreased antibiotic usage, indicating a lag between antibiotic use and ARG wastewater signal.

Publisher

Research Square Platform LLC

Reference74 articles.

1. Cocaine in surface waters: A new evidence-based tool to monitor community drug abuse;Zuccato E;Environ Heal A Glob Access Sci Source,2005

2. Estimating alcohol consumption by wastewater-based epidemiology: An assessment of the correction factor for ethyl sulfate using large-scale national monitoring data;Thai PK;Environ Sci Technol Lett,2021

3. The potential of wastewater-based epidemiology as surveillance and early warning of infectious disease outbreaks;Mao K;Curr Opin Environ Sci Heal,2020

4. CDC. National wastewater surveillance system [Internet]. Centers for Disease Control and Prevention. 2022. Available from: https://www.cdc.gov/healthywater/surveillance/wastewater-surveillance/wastewater-surveillance.html

5. Urban monitoring of antimicrobial resistance during a COVID-19 surge through wastewater surveillance;Harrington A;Sci Total Environ,2022

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3