Association of lncRNA EIF3J-AS1 with target gene IGFB3 polymorphisms in susceptibility of gastric cancer

Abstract

Abstract LncRNA EIF3J-AS1 has shown to play an important regulatory role in a variety of tumor tissues, IGFB3 Gene is the target gene of LncRNA EIF3J-AS1, experimental studies shows that IGFB3 gene risk typing is associated with an increased risk of gastric cancer susceptibility of gastric cancer(GC),the relationship between LncRNA EIF3J-AS1 target IGFB3 Gene polymorphisms expression in GC patients is related. Methods: A case-controlled study was conducted, including 490 primary gastric cancers and 1476 normal controls. Targetscan, miRanda and other software are used to target and predict the genes and mirnas that lncRNA can bind to, and the expression correlation analysis is conducted, so as to construct the ceRNA network and speculate its regulation of gastric cancer expression. The target gene IGFB3 fragment was amplified in blood samples using PCR. Genotyping was performed using the snapshot method. Results: This rearch shows a signal pathway LncRNA EIF3J-AS1-IGFBP3, in IGFBP3 gene variants GA and GA + AA models, castric cancer can be decreased when in male subgroup and ≥ 61 years old subgroup, but during smoking and alcohol groups, gastic cancer risk is encreased. IGFBP3 gene is regulated by LncRNA EIF3J-AS1-miRNAs-IGFBP3 network, which could provide a potential drug target biomarker.