Tension load affects MYOZ2 expression and mediates paraspinal myofiber transformation in adolescent idiopathic scoliosis-Reference-Cited by-同舟云学术

Tension load affects MYOZ2 expression and mediates paraspinal myofiber transformation in adolescent idiopathic scoliosis

Published:2024-06-13 Issue: Volume: Page:
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Author:

Gao Yuan¹,Ying Jia¹,Lai Bowen¹,Jiang Heng¹,Zhou Xuhui¹

Affiliation:

1. Changzheng Hospital, Second Affiliated Hospital of Second Military Medical University

Abstract

Objective To investigate the difference of typeⅠand typeⅡmyofibers of paraspinal muscles between the concave and convex side of main thoracic curve in adolescent idiopathic scoliosis (AIS) and its regulatory mechanism. Methods The paraspinal muscle samples of 46 patients with AIS were collected and stained with ATPase. The myofiber parameters were measured and compared between the concave and convex side and among different vertebrae. The correlation between myofiber parameters and clinical data of the patients was analyzed. The paraspinal muscle samples of 5 AIS patients were selected for transcriptome RNA sequencing and RT-qPCR plus Western-blot were used to verify the results. Meanwhile, the correlation between the mRNA expression and myofiber parameters were analyzed. Finally, The expression of microRNAs between the concave and convex side were screened by microRNA sequencing and verified by RT-qPCR. In addition, the correlations between the microRNAs expression and myofiber parameters were analyzed to explore the possibility of microRNA regulating myofiber transformation. Results There was transformation between typeⅠ and typeⅡ myofibers especially in the apical vertebrae region. Although the transformation may be a secondary change under the different tension load, it is closely related to the progress of curvature in AIS. The expression of MYOZ2 on the convex side was significantly different from that on the concave side, and the MYOZ2 expression was closely related to myofiber parameters.There was a significant difference in the expression of miR-499-5p and miR-133a-3p between the concave and convex side, and the differences of miR-499-5p and miR-133a-3p expression were closely related to to myofiber parameters. Conclusion In AIS patients, the tension load was higher on the convex side and lower on the concave side, which caused an increased expression of miR-499-5p and miR-133a-3p in the paraspinal muscles on the convex side and a decrease expression on the concave side, which inhibited the expression of MYOZ2 on the convex side and promoted the expression of MYOZ on the concave side, and ultimately resulted in the transformation of type II myofibers to type I on the convex side and the transformation of type I to type II on the concave side.

Publisher

Springer Science and Business Media LLC

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