Annona muricata callus extracted particles (AMCEP) as a potential anticancer agent against Human Colorectal Cancer cell line HCT-116 by inducing apoptosis

Abstract

Abstract Although the advances in chemotherapy, radiotherapy and surgery, the mortality and morbidity rate of cancer cases is still high. The use of natural plant derived medicinal product has an emerging modern therapy in order to improve human health and reducing the risk of side effects from synthetic drugs. The aim of this study to evaluate the anticancer potential of Annona muricata callus extracted particles against human colorectal cancer cell line HCT-116. A. muricata callus extracted particles were screened for the cytotoxic activity by MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) assay against HCT-116 & WI-38 cell line. Further, Annexin V apoptosis assay were done by the flow cytometry analysis. The particles from ethanolic extract of A. muricata callus showed cytotoxic effect against HCT-116 cell line with IC50 60.64 µg/ml while it showed mild toxicity only at highest concentration against normal WI-38 cell line. Further, Annexin V apoptosis assay reveals the sample treated cell population of HCT-116 followed the process of early apoptosis as compared to the control cells (untreated). It has been concluded that A. muricata callus extracted particles have potential anticancer agent against human colorectal cancer cell line HCT-116.