Dried Raw Camel Milk Spot “DRCMS” as a simple and efficient microsampling method from hot and remote regions for mesophilic aerobes count and Lactofermentation microbiota activity detection

Abstract

Abstract We tested the validity of the “DRCMS” as new method for the collection and procurement of raw camel milk samples from the field. 100 µl of whole fresh raw camel milk were spotted on Sterile Whatman Filter Paper (SWFP) then, stored at 24°C and 37°C from 0 to 3 days. We compared the log10 cfu/mL count of mesophilic aerobes (MA) populations and the fermenting ability from freshly collected milk and corresponding samples that were spotted on SWFP. Considering the results using parametric unpaired t-test, absence of significant differences (p > 0.05) between means log10 cfu/mL from fresh raw camel kept at 06°C according the conventional method, and those from DRCMS as new storage method were recorded for 85% samples. Besides Lactofermentation test reveal that biological activities of the microbiota in each fresh raw camel milk and its correspondent DRCMS were the same, showing an effective microbiota composition preservation on SWFP.