Pathogen Analysis of Mumps-like Cases Using Metagenomic Nanopore Sequencing Technology

Abstract

Abstract The detection rate of mumps virus in specimens from clinical diagnostic cases of Mumps is generally low. In order to understand the possible pathogens of Mumps-like cases, this study examined the possible pathogens in parotid throat swabs from 22 Mumps-like cases (Case group) and 17 healthy individuals (Control group) by Metagenomic nanopore sequencing technology. For the suspected pathogens found between Case group and Control group, real-time fluorescent quantitative polymerase chain reaction （qPCR）was used for detection and confirmation. At the same time, SPSS 23.0 software was used to statistically compare the significant difference of two groups. It was no significant difference about the detection result of pathogenic bacteria were found in two groups by metagenomic sequencing. Human parainfluenza virus 3(HPIV-3) was detected only in 5 cases in the former, while human herpesvirus 7(HHV-7) was detected in 4 and 6 cases in Case group and Control group, respectively. Further statistical analysis revealed that the difference in the HPIV-3 detection rate between Case group and Control group was statistically significant (X2=9.186, P=0.011), while the HHV-7 detection rate was not statistically significant (X2=0.364, P=0.393). These findings suggested that HPIV-3 may be available pathogen responsible for Mumps-like cases in the investigation area, and attention should be paid to the differential diagnosis and reporting of Mumps, and etiological surveillance and detection should be strengthened.