Integrated analysis of microRNA and messenger RNA expression profiles reveals functional microRNA in infectious bovine rhinotracheitis virus-induced mitochondrial damage in Madin-Darby bovine kidney cells

Abstract

Abstract Background Studies have confirmed that Infectious bovine rhinotracheitis virus (IBRV) infection induces mitochondrial damage. MicroRNAs (miRNAs) are a class of noncoding RNA molecules, which are involved in various biological processes and pathological changes associated with mitochondrial damage. It is not known whether miRNAs participate in IBRV-induced mitochondrial damage in Madin-Darby bovine kidney (MDBK) cells. Results In the present study, we used high-throughput sequencing technology, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis to screen mitochondria-related miRNAs and messenger RNAs (mRNAs). In total, 279 differentially expressed miRNAs and 832 differentially expressed mRNAs were identified in 6 hours (IBRV1) versus 24 hours (IBRV2) after IBRV infection in MDBK cells. GO and KEGG enrichment analysis revealed that 42 differentially expressed mRNAs and 348 target genes of differentially expressed miRNAs were correlated with mitochondrial damage, and the miRNA-mitochondria-related target genes regulatory network was constructed to elucidate their underlying regulatory relationships. The expression patterns of eight of ten differentially expressed miRNAs were consistent with the high-throughput sequencing results, Functional validation results showed that the overexpression of miR-10a and miR-182 aggravated mitochondrial damage and that the inhibition of miR-10a and miR-182 alleviated mitochondrial damage. Conclusions This study revealed not only the expression changes of miRNAs and mRNAs in IBRV-infected MDBK cells but also the possible biological regulatory relationship between them. MiR-10a and miR-182 have the potential to be developed as biomarkers for IBRV diagnosis and treatment. Together, these data and analyses provide additional insight into the role of miRNAs and mRNAs in IBRV-induced mitochondria damage.