Pathogenicity of mycoproteins of Beauveria bassiana and Metarhizium anisopliae to Bactrocera zonata

Abstract

AbstractThe current study revealed the potential release of mycoproteins ofB. bassianaandM. anisopliaeand their lethal effects onB. zonata.After inoculation and precipitation of crude proteins in 95% (NH4)2SO4at 25° C, the crude samples were subjected to SDS-PAGE. The gel electrophoresis analysis revealed the presence of low molecular weight bands of 11kDa and 25kDa proteins fromB. bassianaandM. anisopliaerespectively. The pathogenicity of mycoproteins fromB. bassianaandM. anisopliaewas evaluated when second instar larvae, 3day old pupae and 3day old adults were subjected to protein concentrations. The larval bioassays revealed that significant maximum mortality of 73.3±3.3 and 86.6±3.3 was recorded at interval of 7 days when fed @ 10µl/g ofBbandMaproteins respectively. Low adult emergence of 33.3±4 and 37.5±5.3 was observed when 3day old pupae were treated with proteins @ 30µl ofBbandMaat an interval of 10 day. Low mortality of 38.0±3.7 and 48.0±5.8 was recorded when 3day old adults (♂and♀) were exposed to 30 μl protein fromBbandMaat an interval of 10 days respectively. The findings of the study conclude the production of proteins and their pathogenic effects. The melanization of the larval tissues, abnormal adult emergence and deformation in wing structures were found symptomatic after ingestion of the proteins. However, the purification process of these proteins, their persistence with respect to environmental regimes and their evaluation under the field condition further are needed to be investigated.