Affiliation:
1. Mazandaran University of Medical Sciences
Abstract
Abstract
Introduction: considering the increase in the use of Chlorine-based disinfectants and the questions about the shelf-life of chlorine in solutions, its persistence on the surface, etc., the present study investigated the effect of environmental factors on the stability of commercial solutions stored in different conditions.Materials and methods Three brands of commercial Chlorine-based sanitizers (Golrang, Active, and Tage) were tested. Stability of 0.05, 0.5, and 1% solutions with the pH of 5, 7.5, and 10 stored at 4°C, 25°C, and 45°C were assessed using iodometric titration in 0, 15, 30, and 60 days after preparation. 87 solutions were prepared based on Experimental Design and Response Surface Modeling (RSM) the utilization of Box-Behnken factorial design (BBD).Results Based on RSM and BBD, the effects of linear coefficients of all factors were significant (p < 0.05) and there were no statistically significant differences among the three brands (p = 0.307). There was a negative correlation between the free available chlorine and time. Solutions with a higher initial concentration (1%) tend to have lower stability compared with others. The temperature contributed to the instability of the solutions. There was a gradual decrease in the pH of all solutions over time. The lowest stability was seen in neutral pH (7.5).Conclusion Chlorine-based solutions under different storage conditions have different stability times. All factors affect the stability of chlorine in solutions. Therefore, environmental conditions must be considered when using chlorine solutions.
Publisher
Research Square Platform LLC
Reference22 articles.
1. The effects of temperature, concentration, and tissue type on the solvent ability of sodium hypochlorite;ABOU-RASS M;Journal of Endodontics,1981
2. AL-GHEETHI, A., AL-SAHARI, M., ABDUL MALEK, M., NOMAN, E., AL-MAQTARI, Q., MOHAMED, R., TALIP, B. A., ALKHADHER, S. & HOSSAIN, M. S. 2020. Disinfection methods and survival of SARS-CoV-2 in the environment and contaminated materials: a bibliometric analysis. Sustainability, 12, 7378.
3. ASTM 2017. Standard Test Methods of Sampling and Chemical Analysis of Chlorine-Containing Bleaches. D2022. Pennsylvania, United States: American Society for Testing and Materials.
4. The shelf-life of sodium hypochlorite irrigating solutions;CLARKSON R;Australian Dental Journal,2001
5. Influence of ethylenediaminetetraacetic acid on the active chlorine content of sodium hypochlorite solutions when mixed in various proportions;CLARKSON RM;Journal of Endodontics,2011