Abstract
There is a lack of study on the muscular-deep fascia system which connects the upper eyelid, brow, and glabella. This study aimed to explore the fine anatomy of the muscular-deep fascia system at the region between the eyebrow and the superior orbital margin. We included eight formalin–phenol-embalmed cadavers (16 sides of specimens), and categorized them for anatomical dissection and histological sections. Five cadavers (10 sides) were dissected for gross anatomical observation, whereas all soft tissues of the other three cadavers (6 sides) were dissected for tissue sectioning and histological analysis. Three tissue blocks and 16 strips in each block were trimmed, numbered, and sliced in these specimens. Hematoxylin–Eosin and Masson’s Trichrome staining were undertaken respectively. In the region between the eyebrow and the superior orbital margin, the frontalis was covered by the orbicularis oculi. Fibers of the frontalis muscle penetrated into the orbicularis oculi muscle bundles, and crosslinked around the eyebrow level. Both the frontalis and the orbicularis oculi were attached to the thickened multilayered deep fascia in this region, which could be regarded as the muscular-deep fascia system. The muscular-deep fascia system connects the frontalis deep fascia and deep forehead compartments down to the orbicularis-supporting ligament. The precise anatomy of the muscular-deep fascia system at the region between the eyebrow and the superior orbital margin may provide valuable reference for soft-tissue fixation and suspension in facial surgery.