Affiliation:
1. Departement of Clinical Pathology, Faculty of Medicine, Brawijaya University
2. Department of Dermatology and Venereology, Faculty of Medicine
3. Doctoral Program of Medical Science, Brawijaya University
Abstract
Abstract
Background: to analysed the effect of Orthosiphon aristatus on repairing skin lesions by regulating cytokines such as IgE, IL4, IL22, PGE2, NO in DNCB-induced Atopic Dermatitis BALB/C Mice Model
Methods: used BALB/C Mice which were sensitized by DNCB for 21 days to developed atopic dermatitis model. Mice were administered oral Orthosiphon aristatus extract once daily (on last 14 days after 7 days of sensitization). The doses given were divided into 6 groups: 17.5 mg/kgbw, 35 mg/kgbw, 70 mg/kgbw, and 140 mg/kgbw. We analysed the levels of cytokines such as IgE, IL4, IL22, PGE2 obtained from the blood. Additionally, we also measured morphological skin lesion severity to confirmed the amelioration effect clinically.
Results: Administration of Orthosiphon aristatus extract reduced the skin lesion severity in all intervention groups. The clinical improvement was supported by decrease of IgE, IL4, IL22, and PGE2 in dose dependent manner.
Conclussion: Orthosiphon aristatus alleviates DNCB-induced Atopic Dermatitis BALB/C Mice Model clinically through downregulating of IgE, IL4, IL22, PGE2, NO levels.
Publisher
Research Square Platform LLC
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