Preparation of high temperature and low temperature resistant solid microbial agent for cattle manure fermentation and effect on composting

Abstract

Abstract Objective: We used microbiology and molecular biology techniques to screen out high temperature and low temperature resistant saprobiotics for compost and prepared a compound fermentation bacteria agent to rapidly ferment cattle manure into high-quality organic fertilizer in low temperature season. Methods: Conventional composting and high-throughput techniques were used to analyze the changes of physical and chemical indexes and biodiversity in the process of composting, from which high and low temperature resistant strains were obtained, and high temperature and low temperature resistant solid composite bactericides were prepared and added to composting to verify the effects of composite bactericides on composting. Results: The conventional composting cycle took 22 days, and the diversity of microflora increased first and then decreased. Composting temperature and microbial population were the key factors for the success or failure of composting. Two strains of high temperature tolerant bacteria and 6 strains of low temperature resistant bacteria were screened out and they were efficient in degrading starch, cellulose and protein. The high temperature and low temperature resistant solid bacterial agent were successfully prepared with adjuvant. The preparation could make the compost temperature rise quickly at low temperature, the high temperature lasted for a long time, the water content, C/N and organic matter fell quickly, and the contents of total phosphorus and total potassium were increased, and the seed germination index was significantly improved. Improve the composting effect. Conclusion: The solid composite bacterial agent can shorten the composting time at low temperature, and improve the composting efficiency and quality.