Abstract
Inter-root microorganisms are major factors influencing the formation of high-quality herbs and promoting the accumulation of secondary metabolites, and this relationship has been well characterised in many supra-herbal medicinal herbs, but there is limited information on whether ginseng saponin accumulation is mediated by microorganisms in different forest types.Inter-root soil samples of ginseng and ginseng samples were collected from four different forest types (Theropencedrymion, Larch forest, Broad-leaved forest and Camphor pine forest) in the mountainous areas of Jingyu County, Jilin Province, China. The content of ginsenosides in the collected ginseng samples was determined by high performance liquid chromatography (HPLC). The results showed that the content of ginsenosides in Camphor pine forest was significantly higher than that in the other three forest types.The microorganisms in the soil samples were isolated and purified, and subsequently sequenced and analyzed by high-throughput sequencing methods, and a total of seven bacterial species were isolated and identified in the inter-root soil of ginseng from four different forest types. In broad-leaved forests (BF) and larch forests (LF), Bacillus megaterium is the most abundant microorganism. In the camphor pine forests (CPF) and theropencedrymion (TH), Luteibactor rhizovicinais the largest proportion of microorganisms. Relevant analysis shows that several identified strains from the four forest types, including Bacillus pseudomycoides, Bacillus subtilis, Pseudomonas alcaliphila, Luteibacter rhizovicinus and Pseudomonas alcaliphilacan promote the biosynthesis and accumulation of monomeric saponins Rc, Rb1, Rb2, Rb3, Rg2, Rb3, and Rh4. Our research findings emphasize the crucial role of different forest stand types in soil microbial community structure, and explore the accumulation mechanism of ginsenosides from a microbial perspective. In summary, this study provides more theoretical basis for the relationship between different forest types and the bioactive components of medicinal plants.