Bioinformatic role of MIR-101 on PTEN and Mcl1 target genes in liver cancer patients

Abstract

Abstract One of the three causes of death in the world is liver cancer, which is common in today's society. One of the new methods in the treatment of liver cancer is the identification of new and less invasive biomarkers, among which microRNA can be mentioned. Therefore, in this study, the bioinformatic role of mir-101 on the target genes PTEN and Mcl1 in liver cancer was evaluated. Materials and methods: In this theoretical study, Target Scan, miR Base, DIANA Tool, miRanda, miRTarBase, miR Walk, miR path target genes and signaling pathway of miR-101 were investigated using bioinformatics databases. Then, GEO data was analyzed with R software to analyze miR-101. Finally, the biomarker role of miR-101 was analyzed with ROC diagram. Results: Investigations conducted using different databases as well as statistical methods using R software and graph prism provided a comprehensive analysis of miR-101-3p/5p and based on the analysis of the ROC diagram, the specificity of miR-101 was reported to be higher than 0.5. Also, investigations and target gene selection from different bioinformatics databases showed that PTEN genes with their tumor suppressive role and Mcl1 with their anti-apoptotic role in the TGF- β signaling pathway had dual behavior against each of the studied target genes. Finally, it inhibited metastasis in the signaling pathway of liver cancer, thus reducing the expression of microRNA in patients with liver cancer. Conclusion: According to the obtained results, miR-101 is a targeted therapy for the diagnosis, prognosis and treatment of HCC.