Sporosarcina siccatus sp. nov., isolated from coastal sediment of the Great Wall Station, Antarctica

Abstract

Abstract A Gram-stain-positive, aerobic and spherical bacterium, white, slightly convex colony with rounded edges, designated GW1-11T was isolated from the coastal sediment of the Great Wall Station, Antarctica. 16S rRNA gene sequence analysis showed that strain GW1-11T was closely related to Sporosarcina ureae DSM 2281T (94%). Growth was observed in the presence of 0–6.0%(w/v) NaCl, at pH 6.0–9.0, and at 4–37℃. Digital DNA-DNA relatedness between strain GW1-11T and its close relatives S. ureae DSM 2281T was 21.0%. The average nucleotide identity value between strain GW1-11T and S. ureae DSM 2281T was 77.9%. The strain GW1-11T possessed menaquinone-7 (MK-7) as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids composition was summarized as C14: 0, C14:0 ω5c and iso-C14: 0. Based on phenotypic, chemotaxonomic characteristics, and phylogenetic data, strain GW1-11T represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siccatus sp. nov., is proposed. The type strain is GW1-11T (= CCTCC AB 2019391T = KCTC43187T).