A normalized model based on Taqman real-time PCR assay for quantitative comparison of chicken adulteration in raw and heat-treated hamburgers-Reference-Cited by-同舟云学术

A normalized model based on Taqman real-time PCR assay for quantitative comparison of chicken adulteration in raw and heat-treated hamburgers

Published:2022-08-10 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Sarlak Zahra¹,Rezvani Nayebali¹,Rouhi Milad¹,Shojaee-Aliabadi Saeedeh²,Hosseini Hedayat²

Affiliation:

1. Kermanshah University of Medical Sciences

2. National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences

Abstract

Abstract Mislabeling of hamburgers with undeclared chicken has frequently been reported. A fast TaqMan real-time PCR assay was developed to estimate chicken percentage in raw and heat-treated hamburgers in the current work. The calibration curve was plotted with the normalization approach (ΔCt; the difference calculation between chicken-cytb and eukaryotic-18S rRNA assays) for five reference chicken mixtures (100-0.01%) using a duplex TaqMan-qPCR protocol. The R2 and efficiency values of the normalized curve were 0.9993 and ~100%, respectively. In the validation plan, analysis of model samples (raw, oven-cooked and autoclaved) under repeatability conditions represented the bias values within the range of ±25% and RSD values <25%, except for a few autoclaved model samples. Therefore, this developed and validated model could be considered a useful and reliable method for estimating chicken species (with a sensitivity of 0.01%) in raw and cooked hamburgers.

Publisher

Research Square Platform LLC

Reference33 articles.

1. Screening of commercial meat products from supermarket chains for feline derivatives using SP-PCR-RLFP and lab-on-a-chip;Al Amin M;Journal of Food Composition and Analysis,2020

2. Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products;Amaral JS;Food control,2017

3. Bustin SA et al. (2009) The MIQE Guidelines: M inimum I nformation for Publication of Q uantitative Real-Time PCR E xperiments. Oxford University Press,

4. A Simple and Reliable Single Tube Septuple PCR Assay for Simultaneous Identification of Seven Meat Species;Cai Z;Foods,2021

5. Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures;Cammà C;Food Control,2012