The Transcriptome Induced by Bazhen Decoction and its Function in G-quadruplex Resolving and Telomere Maintenance in Progeroid Cells

Author:

Li Chuanbiao1,Tian Jie1,Yang Jun1,Wang Qianqian1,Guo Bing1,Huang Liming2,Hu Ying1,Luo Ying1ORCID

Affiliation:

1. Guizhou Medical University

2. Guizhou University Of Traditional Chinese Medicine

Abstract

Abstract Background The Bazhen decoction is one of the most extensively used Traditional Chinese medicine (TCM) prescriptions for anti-aging. However, due to the complicity of the components, the pharmacological mechanism of Bazhen decoction is still limited.Methods We applied RNA sequencing and transcriptome analysis to get the full view of the signaling pathways regulated by Bazhen decoction in the wild type cell background. By using the progeroid cells derived from Werner syndrome mice, we applied Western blot, Immunofluorescence, flow cytometry and telomere FISH to verify the transcriptome data.Results The transcriptome profile revealed that Bazhen decoction might systematically regulate multiple anti-aging pathways, including stem cell regulation, protein homeostasis, cardiovascular function, neuronal function, anti-inflammation, anti-DNA damage induced stress, DNA helicase activity and telomere lengthening. We found that multiple DNA helicases and telomere regulating proteins were up-regulated by Bazhen decoction, which promoted the resolving of G-quadruplex (G4) structure, and facilitated DNA replication and telomere elongation. These improvements also endowed the cellular resistance to DNA damages induced by replication stress. Together these data suggest that Bazhen decoction facilitate G4 resolving and telomere maintenance, which might contribute to the longevity sustaining properties revealed by transcriptome profile.Conclusions Our data revealed a new strategy for recovering the pharmacological signature pathways for TCM, which could help the clinical precision medicine of TCM. By applying transcriptome in TCM-treated normal cell, we tried out a systematic analysis for dissecting the molecular mechanism of complicated TCM prescription in the normal genetic background.

Publisher

Research Square Platform LLC

Reference35 articles.

1. [Chuzhen therapy for sub-health: a randomized controlled study];Hu YP;Zhongguo Zhen Jiu,2012

2. [Comparison of the effect of sijunzi decoction, siwu decoction and bazhen decoction on immune function in mice];Xu AH;Zhongguo Zhong Yao Za Zhi,1993

3. Beneficial Effects of Traditional Chinese Medicine in the Treatment of Premature Ovarian Failure;Li M;Evid Based Complement Alternat Med,2022

4. [Clinical study of Jiawei Bazhen decoction combined with oxytocin for cervical ripening of qi and blood deficiency type of pregnant women];Ke XY;Zhongguo Zhong Yao Za Zhi,2015

5. Effects and mechanisms of Bazhen decoction, Siwu decoction, and Sijunzi decoction on 5-fluorouracil-induced anemia in mice;Tian Y;J Tradit Chin Med,2016

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