TRβ gene expression of Sprague- Dawley rats’ hypothyroidism treated with non-Protein amino acid beta-amino butyric acid (βABA)

Abstract

Abstract Thyroid receptors TRα, TRβ, and other receptor subunits belong to the nuclear receptor family as they mediate thyroid hormone signaling to regulate a wide range of physiological processes including growth and metabolism. Our study aimed to investigate the effect of beta-aminobutyric acid (βABA) on the gene expression of the TRβ gene in 30 male Sprague-Dawley rats with hypothyroidism and normal condition. Animals were divided into six groups, three of which developed hypothyroidism by intraperitoneal injection with propylthiouracil (PTU) daily for 15 days, while the other three groups did not develop hypothyroidism and remained healthy. Then four groups of rats (two infected and two normal groups) were injected with two concentrations of (βABA) (100 and 200 mg/kg body weight) three times a week for two weeks. An infected control group was left for βABA treated groups and a normal control group was left for all groups. The gene expression level of the TRβ gene was measured, and it was found that there was a decrease in relation to the infected control group only while the expression value increased in the infected and treated groups treated with amino acid in both concentrations and with regard to the normal and injected groups with (βABA), the gene expression increased in the group injected with a concentration of 100 mg/kg only. We conclude that BABA had a positive role in regulating the secretion of pro-and anti-inflammatory cytokines under studied concentrations besides it had a positive role in increasing the expression of the TRβ gene.