Affiliation:
1. Shijiazhuang Obstetrics and Gynecology Hospital
Abstract
Abstract
Background
The incidence rate of gestational diabetes mellitus (GDM) is still high among pregnant women in the second trimester of pregnancy. This study explored the therapeutic effects of omega-3 fatty acids (ω-3 FAs) on GDM at the cellular and animal levels.
Methods
THP1 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) to induce M0 macrophage. The transformation of M0 macrophages into M2 macrophages was observed after ω-3 FAs treatment. Then, macrophages induced by ω-3 FAs were co-cultured with hepatocytes HepG2, and the glycolipid metabolism in hepatocytes was assessed. By establishing a GDM mouse model, the impact of ω-3 FAs on liver function in GDM pregnant mice and offspring was evaluated.
Results
At the cellular level, we found that ω-3 FAs can promote the transformation of M0 macrophages into anti-inflammatory M2 macrophages, and the transformed M2 macrophages can prevent excessive accumulation of lipid droplet in hepatocyte cell line HepG2, by promoting β-oxidation and reducing lipid synthesis of hepatocyte, thereby protecting hepatic function. Supplementation of ω-3 FAs in pregnant GDM mice significantly reduced fasting blood glucose levels, GTT and ITT indexes, and lipid accumulation in the liver, and effectively prevented liver fibrosis. ω-3 FAs also had positive effects on the offspring of GDM pregnant mice, demonstrated by reducing birth mortality and improving glycemic stabilization.
Conclusion
This study suggests that ω-3 FAs prevent GDM via modulating lipid metabolism and may provide a strategy for translational medicine that can treat GDM and offspring.
Publisher
Research Square Platform LLC
Reference23 articles.
1. Gestational Diabetes Mellitus Prevalence, Screening, and Treatment Practices in American Samoa, 2016;Meyer BN;Hawaii J Health Soc Welf,2022
2. Prevalence of gestational diabetes in the Waikato region of New Zealand;Chepulis L;Intern Med J,2022
3. Prevalence and factors associated to gestational diabetes mellitus among pregnant women in Libreville: a cross-sectional study;Lendoye E;Pan Afr Med J,2022
4. Prevalence and Risk Factors of Gestational Diabetes Mellitus in Bangladesh: Findings from Demographic Health Survey 2017–2018;Mazumder T;Int J Environ Res Public Health,2022
5. Prevalence of Gestational Diabetes Mellitus among pregnant women attending antenatal care clinic of St. Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia;Nigatu B;Clin Diabetes Endocrinol,2022