Affiliation:
1. Karolinska Institutet
2. University of Cadiz
3. Massachusetts General Hospital, Harvard Medical School
Abstract
Abstract
Deciphering the striatal interneuron diversity is key to understanding the basal ganglia circuit and to untangle the complex neurological and psychiatric diseases affecting this brain structure. We performed snRNA-seq of postmortem human caudate nucleus and putamen samples to elucidate the diversity and abundance of interneuron populations and their transcriptional structure in the human dorsal striatum. We propose a new taxonomy of striatal interneurons with eight main classes and fourteen subclasses and provide their specific markers and some quantitative FISH validation, particularly for a novel PTHLH-expressing population. For the most abundant populations, PTHLH and TAC3, we found matching known mouse interneuron populations based on key functional genes such as ion channels and synaptic receptors. Remarkably, human TAC3 and mouse Th populations share important similarities including the expression of the neuropeptide tachykinin 3. Finally, we were able to integrate other published datasets supporting the generalizability of this new harmonized taxonomy.
Publisher
Research Square Platform LLC
Reference75 articles.
1. Fix, J.D. (2008). Neuroanatomy: includes online access to full text and questions from the book! 4th ed. (Wolters Kluwer, Lippincott Williams & Wilkins).
2. The two-century journey of Parkinson disease research;Przedborski S;Nat Rev Neurosci,2017
3. Striatal synaptic adaptations in Parkinson’s disease;Shen W;Neurobiology of Disease,2022
4. Brain atrophy in Alzheimer’s Disease and aging;Pini L;Ageing Research Reviews,2016
5. Transcriptional vulnerabilities of striatal neurons in human and rodent models of Huntington’s disease;Matsushima A;Nat Commun,2023
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