Affiliation:
1. Beijing university of chemical technology
2. Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
3. University of Helsinki
4. Chinese Academy of Sciences
5. Chalmers University of Technology
Abstract
AbstractCO2fixation plays a key role to make biobased production cost competitive. Here, we used 3-hydroxypropionic acid (3-HP) to showcase how CO2fixation enabled approaching theoretical-yield production. Using genome-scale metabolic models to calculate the production envelope, we demonstrated that the provision of bicarbonate, formed from CO2, sealed previous attempts for high yield production of 3-HP. We thus developed multiple strategies for bicarbonate uptake, including the identification of Sul1 as a bicarbonate transporter, domain swapping and engineering of malonyl-CoA reductase, identification of Esbp6 as a 3-HP exporter, and deletion of Uga1 to prevent 3-HP degradation. The combined rational engineering increased 3-HP production from 0.15 g/L to 11.25 g/L in shake-flask using 20 g/L glucose, approaching the maximum theoretical yield with concurrent biomass formation. The engineered yeast forms the basis for commercialization of bio-acrylic acid, while our CO2fixation strategies pave the way for CO2being used as the sole carbon source.
Publisher
Research Square Platform LLC