Cohesive transcriptomic and in vitro approach revealed genes involved in inhibition of G0/G1, G2 phase of cell cycle pathway of stomach cancer cells by geraniol treatment

Abstract

Abstract The effects of geraniol on cell cycle related pathway in AGS using RNA sequencing have not been explored and it is largely unknown. In this study, we isolated geraniol from Cymbopogon martini (palmarosa) essential oil using various spectroscopic analyses. At first, we carried out the cytotoxicity of geraniol on AGS cells. In-depth RNA sequencing analysis showed that geraniol negatively regulated genes that specifically initiate double-strand break repair via DNA replication, mitotic G1, G2/M transition, and S phases in cell cycle, eventually leading to induce apoptosis. Additionally, we validated the interaction of geraniol with the cell cycle related genes using docking, Florescence-activated cell sorting (FACS) and quantitative polymerase chain reaction (qPCR) analysis. Overall, the present investigation shows that geraniol interacts with specific target genes involved in the cell cycle process and induce cell death in the stomach cancer cells, which can be suggested as the potential treatment for stomach cancer.