Abstract
Abstract
Background
Lactic Acid Bacteria (LAB) plays an important role in the fermentation of traditional food of ethnic communities across the globe. This study isolated and identified LAB from ‘Pakkat su’, vegetable-based fermented food of the Tai Phake community of Assam. Pure microbial cultures were screened for their probiotic properties, viz., tolerance to acid and bile, bile salt hydrolysis, resistance against antibiotics, antimicrobial and hemolytic activities, and cell surface characteristics.
Result
Three isolates from pure cultures showed bile, pH and bile tolerance and non-hemolytic and non-pathogenic properties. Antagonistic effects of these isolates were observed against E.coli ATCC-10536 and Staphylococcus aureus ATCC-BAA-1026. These potent bacterial isolates were identified as new strains of Lactiplantibacillus plantarum, Lactobacillus casei, and Lactiplantibacillus pentosus based on 16SrRNA sequences which were submitted to GenBank database with sequential accession number OQ996876, OQ997380 and OQ998330.
Conclusion
These results indicate that microbes associated with ‘Pakkat su’ production are good candidates for probiotic production and can be considered for potential application as commercial probiotic to confer health benefits to consumers. It also implies that ‘pakkat su’ can be used as vehicles for probiotic delivery.
Publisher
Research Square Platform LLC
Reference60 articles.
1. FAO (1998) Fermented Fruits and Vegetables-A Global Perspective, vol 134. FAO Agricultural Services Bulletin, Rome, Italy
2. Antagonistic activities of lactic acid bacteria in food and feed fermentations;Lindgren SE;FEMS Microbiol Rev,1990
3. Bacteriocins from lactic acid bacteria: production, purification, and food applications;Vuyst L;J Mol Microbiol Biotechnol,2007
4. Phenotypic and genotypic identification of lactic acid bacteria isolated from ethnic fermented bamboo tender shoots of North East India;Tamang B;Int J Food Microbiol,2008
5. Metchnikoff (1908) The prolongation of life: optimistic studies. English trans Mitchell PC.GP Putnam's Sons, New York1908