Orally administered Thymoquinone mitigates cypermethrin-induced dentate gyrus oxidative stress, preventing GABAergic interneuron degeneration and memory impairment in rats via the Nrf2/ARE pathway.

Author:

Imam Abubakar Lekan1,Okesina Akeem Ayodeji2,Sulamon Fatimo Ajoke1,Imam Aminu1,Ibiyeye Ruqayyah Yetunde3,Oyewole Lukuman Aboyeji1,Biliaminu Sikiru Abayomi1,Shehu Monsur1,Abdulhameed Alli Oluwatomi1,Omoola Oluwatosin Olasheu4,Ajao Salihu Moyosore1

Affiliation:

1. University of Ilorin

2. University of Rwanda

3. Kwara State University

4. Kampala International University

Abstract

Abstract Background Exposure to chemical toxins, including insecticides, has harmful effects on body organs such as the brain. This study examined the neuroprotective of thymoquinone on the cypermethrin's harmful effects on the histoarchitecture of the dentate gyrus as well as motor deficit. Methods Forty adult male rats (180-200g) were randomly divided into 5 groups (n = 8 per group). Groups I, II, III, and IV received oral administration of 0.5 ml of phosphate buffered saline, 20 mg/kg of cypermethrin, 10 mg/kg of thymoquinone, 20 mg/kg cypermethrin plus 5 mg/kg of thymoquinone, and 20 mg/kg of cypermethrin plus 10 mg/kg of thymoquinone for 14 days respectively. The novel Object recognition test assesses intermediate-term memory at days 14 and 21 of the experiment. At the end of these treatments, the animals were euthanized and taken for cytoarchitectural analysis and immunohistochemical studies. Result The study shows that thymoquinone at 5 and 10 mg/kg improved Novelty preference and discrimination index. Thymoquinone enhanced Nissl body integrity, increased GABBAergic interneuron expression, nuclear factor erythroid 2-derived factor 2, and enhanced Bcl-2 expression in the dentate gyrus. It also improved the concentration of nuclear factor erythroid 2-derived factor 2, increased the activities of superoxide dismutase and glutathione, and decreased the concentration of malondialdehyde level against cypermethrin-induced neurotoxicity. Conclusion thymoquinone could be a therapeutic agent against cypermethrin poisoning.

Publisher

Research Square Platform LLC

Reference48 articles.

1. Role of mitochondrial ROS in the brain: from physiology to neurodegeneration;Angelova PR;FEBS Lett,2018

2. Progesterone mitigates trimethyltin-induced hippocampal damage through oxidative stress biomarkers;Okesina A;Trop J Health Sci,2022

3. Caspases in cell death, inflammation, and disease;Opdenbosch N;Immunity,2019

4. Cell Death Signaling;Green DR;Cold Spring Harb Perspect Biol,2015

5. Activation of pro-apoptotic cells, reactive astrogliosis and hyperphosphorylation of tau protein in trimethyltin-induced hippocampal injury in rats;Okesina AA;Anat J Afr,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3