Affiliation:
1. Nagoya City University Graduate School of Medical Sciences
Abstract
Abstract
In isolated segments of the rat proximal colon, the dopamine reuptake inhibitor GBR 12909 (GBR) causes dilatation, while the D1-like receptor antagonist SCH 23390 (SCH) causes a tonic constriction, suggesting that neurally-released dopamine tonically stimulates enteric inhibitory efferent neurons. Here, the targets of the enteric dopaminergic neurons were investigated. Cannulated segments of rat proximal colon were bathed in physiological salt solution and luminally perfused with 0.9% saline, while all drugs were applied to the bath. Spatio-temporal maps of colonic motility were constructed from video recordings of peristaltic contractions in the colonic segments, and the maximum segmental diameter was measured. GBR (1 µM)-induced dilatations of colonic segments were prevented by SCH (5 µM), L-nitro arginine (L-NA; 100 µM), a nitric oxide synthase inhibitor, or tetrodotoxin (0.6 µM). In contrast, constrictions induced by a higher concentration of SCH (20 µM) were not prevented by either L-NA or tetrodotoxin. The vasoactive intestinal peptide (VIP) receptor antagonist VIP10 − 28 (3 µM) or P2Y1 receptor antagonist MRS 2500 (1 µM) had no effect on either the GBR-induced dilatation or the SCH-induced constriction. In colonic segments isolated from the rats that had been pretreated with 6-hydroxydopamine to deplete enteric dopamine, GBR failed to increase the colonic diameter, while SCH was still capable of constricting colonic segments. Enteric dopaminergic neurons appear to project to nitrergic neurons to dilate the proximal colon by activating neuronal D1-like receptors. In addition, constitutively activated D1-like receptors expressed in cells yet to be determined may provide a tonic inhibition on colonic constrictions.
Publisher
Research Square Platform LLC