Ag+ ions are effective elicitors for enhancing the production of phenolic acids and tanshinones in Salvia aristata Aucher ex Benth. hairy roots

Author:

Rahchamani Raziey1,Radjabian Tayebeh2ORCID,Abrishamchi Parvaneh3

Affiliation:

1. Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

2. Shahed University

3. Department of Biology, Faculty of Science, Ferdowsi Mashhad, Mashhad, Iran

Abstract

Abstract

Salvia aristata Aucher ex Benth., due to containing tanshinones and phenolic acids, two main groups of bioactive metabolites, is one of the most valuable medicinal plant species in Iran. In this study, for the first time, hairy root (HR) cultures were established from 14-day-old seedlings inoculated with Agrobacterium rhizogenes strain ATCC 15834. Additionally, the effects of elicitation with Ag+ ions (15 and 25 µM) were investigated on the growth indices and content of phenolic acids and tanshinones of HRs in a time-course experiment. The findings of this work showed that exposure of HRs to Ag+ at both concentrations caused significant increases in the levels of rosmarinic acid (1.34- to 1.43-fold of control) and salvianolic acid B (1.71- to 1.82-fold of control). Specifically, 7.25-, 7.78-, 6.47-, and 3.9-fold increases were attained in total tanshinone, tanshinone I, tanshinone II-A, and cryptotanshinone contents of HRs, respectively, after seven days of exposure to 25 µM Ag+ ions compared to the control groups. The analysis revealed that exposure to Ag+ ions significantly enhanced the secretion of tanshinones, notably tanshinone II-A (31.49 ± 0.65 µg mL− 1) by HRs into the culture medium. The changes in transcript levels of crucial genes in the biosynthesis pathways of phenolic acids (PAL, TAT, and RAS) and tanshinones (CPS and CYP76AH1) were associated with their contents in HRs under elicitation with Ag+ ions. Our findings confirmed the effectiveness of an elicitation strategy to improve metabolite production in HR cultures of S. aristata as potent natural sources of phenolic acids and tanshinones.

Publisher

Springer Science and Business Media LLC

Reference61 articles.

1. Triterpenic acid content and cytotoxicity of some;Abdollahi-Ghehi H;species Iran Nat Prod Commun,2019

2. Atabaki N, Shaharuddin NA, Ahmad SA, Nulit R, Malik S, Vahedi M, Kalhori N, Abiri R (2024) Hairy root culture: a reliable bioreactor from transgenic plants. In: Jain A, Malik S (eds) Peptide and protein drug delivery using polysaccharides. Academic Press is an imprint of Elsevier, London, pp 25–50. https://doi.org/10.1016/B978-0-443-18925-8.00013-1

3. Elicitor-induced phenolic acids accumulation in Salvia virgata Jacq. hairy root cultures;Attaran Dowom S;Plant Cell Tiss Org (PCTOC),2022

4. Bayesteh B, Abrishamchi P, Asili J, Zaker A (2021) In vitro production of tanshinones in adventitious root cultures of Perovskia abrotanoides Kar. under Ag+ ion and yeast extract elicitation. J Plant Res 34:139–151. 20.1001.1.23832592.1400.34.1.11.8

5. A new record for Turkey: Salvia aristata Aucher ex Benth. (Lamiaceae);Behçet L;Turk J Bot,2009

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3