The Role of Reduced Glutathione on the Activity of Adenosine Deaminase, Antioxidative System, Aluminum and Zinc Levels in Experimental Aluminum Toxicity

Abstract

Abstract Aluminum (Al) is the most common and widely used element in nature, which cause many health problems and toxic effects. The accumulation of aluminum in the brain, which easily crosses the blood-brain barrier, causes neurodegenerative diseases such as Alzheimer's and dementia, especially diseases associated with free radicals. Reduced glutathione (GSH) is an intracellular tripeptide with low molecular weight, hydrophilic properties, and strong antioxidant effects. This study aimed to investigate the role of reduced glutathione on the activity of adenosine deaminase, antioxidative system, aluminum and zinc levels in aluminum toxicity. In this study, Sprague Dawley rats (n=32) were used. The rats were divided into four equal groups. Group I received 0.5 mL intraperitoneal injection of 0.9 % saline solution (NaCI), Group II received single-dose AlCI3 , Group III was given GSH for seven days, Group IV was given AlCI3 single dose and at the same time 100 mg/kg GSH was given for seven days At the end of the experimental period of seven days, blood samples were collected by cardiac puncture. Plasma TAS and Zn levels were lower than the control group. In contrast, plasma TOS and aluminum concentrations and ADA activity were higher in the aluminum-administered group than in the control group. It was determined that the changed parameters returned to their normal levels in the group given GSH together with aluminum. As a result, additional GSH administration has a protective effect on adenosine deaminase activity, antioxidant system, and Zn level in experimental aluminum toxicity.