Affiliation:
1. Kavian Institute of Higher Education
2. Mashhad University of Medical Sciences
3. Sheffield Hallam University
4. Sahand University of Technology
5. Ferdowsi University of Mashhad
Abstract
Abstract
One of the causes of colon and gastric cancer is the regulation of carcinogenic genes, tumor inhibitors, and micro-RNA. The purpose of this study is to apply rs10811661 polymorphism in CDKN2A /B gene as an effective biomarker of colon cancer and early detection of gastric cancer. As a result,400 blood samples, inclusive of 200 samples from healthy individuals and 200 samples (100 samples from intestinal cancer,100 samples from stomach cancer) from the blood of someone with these cancers, to determine the genotype of genes in healthful and ill people through PCR-RFLP approach and Allelic and genotypic tests of SPSS software. An observe the connection between gastric cancer and bowel cancer risk and genotypes, the t-student test for quantitative variables and Pearson distribution for qualitative variables have been tested and the results have been evaluated using the Chi-square test. The effects confirmed that the highest frequency of TT genotypes is in infected individuals and CC genotype is in healthful individuals. In addition, it confirmed that women were more inclined than men to T3 tumor invasion and most grade II and III colon cancers, and in older sufferers with gastric cancer, the tumor grade tended to be grade I. Among genetic variety and rs10811661, with invasiveness, there is a tumor size and degree in the affected person. In summary, our findings suggest that the rs10811661 polymorphism of the CDKN2A / B gene is strongly associated with the occurrence of intestinal cancer and Stomach is linked to its potential role as a prognostic biomarker for the management of bowel cancer and stomach.
Publisher
Research Square Platform LLC
Reference55 articles.
1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 2018, 68, 394–424. [CrossRef] [PubMed]Nagini, S. Carcinoma of the stomach: A review of epidemiology, pathogenesis, molecular genetics, and chemoprevention. World J. Gastrointest. Oncol. 2012, 4, 156–169. [CrossRef]
2. Epidemiology of gastric cancer: Global trends, risk factors, and prevention;Rawla P;Gastroenterol Rev,2019
3. Gastric Cancer: Descriptive Epidemiology, Risk Factors, Screening, and Prevention;Karimi P;Cancer Epidemiol Biomark Prev,2014
4. Distinct molecular subtypes of gastric cancer: From Laurén to molecular pathology;Cisło M;Oncotarget,2018
5. The two histological main types of gastric carcinoma: Diffuse and so-called intestinal-type carcinoma. An attempt at a histo-clinical classification;Lauren P;Acta Pathol Microbiol Scand,1965