Fascin-1 re-expression promotes cell metastasis through epithelial-mesenchymal transition in canine mammary tumour CHMm cell

Abstract

Abstract Background Canine mammary tumour (CMT) is the most common tumour disease in female dogs. At the same time, the popular concept of preferring purebred dogs when choosing pets has affected the incidence of mammary tumours in dogs. In this study, we obtained a metastatic key protein, Fascin-1, by comparing the proteomics data of in situ tumour and metastatic cell lines from the same individual. However, the role of Fascin-1 in CMT cell line is still unclear. Results To investigated the effects and mechanism of re-expression Fascin-1 on the migration, adhesion and invasion using the CMT cell line CHMm in vitro. Firstly, the proteomics and Western blot methods were used to analyse the differential expression of Fascin-1 between CMT cell line CHMm and CHMp. The CHMm cells isolated from CMT abdominal metastases barely express Fascin-1. Then, the Fascin-1 re-expression cell line CHMm-OE was established using Lentivirus transduction. The CHMm-OE cells could enhance the abilities of cell migration, adhesion, and invasion, promote the formation of lamellipodia, and affect the protein expression related to metastasis and EMT proteins that proteomic points out. Finally, the differentially expressed proteins (DEPs) in CHMm and CHMm-OE cells were identified through proteomics, which involved the positive regulation of cell adhesion, epithelial cell differentiation, extracellular matrix, focal adhesion, dioxygenase activity, and cytoskeleton protein binding. Reactive oxygen species, IL-17, VEGF, and HIF-1 signalling pathway along with Fascin-1 might be involved in cell metastasis. Conclusions However, the Fascin-1 re-expressed could promote cell EMT and increase the formation of lamellipodia, resulting in the enhancement of CHMm cell migration, adhesion and invasion in vitro. This may be beneficial to improve the welfare of female dogs and improve the prognosis of CMT.