Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Abstract

Background To investigate the relationship between changes in miR-377-3p and cell cyclin G2 (CCNG2) expression and clinical features of pregnant women in preeclampsia (PE) and to further analyze the effect of miR-377-3p on CCNG2. Methods 120 pregnant subjects were recruited consisting of 70 PE and 50 healthy pregnancies. The expression of miR-377-3p and CCNG2 mRNA were detected through RT-qPCR, and the protein expression of CCNG2 was examined through Western Blot. The Pearson test was used to analyze the relationship between miR-377-3p and CCNG2 mRNA expression and clinical parameters related to pregnant women with PE, and the association between them. Dual luciferase assay to verify the effect of miR-377-3p on CCNG2. Results The expression of miR-377-3p in the peripheral plasma and placenta of pregnant women in the PE group was lower than that in the control group, and the expression levels of CCNG2 mRNA and protein were higher than that in the control group. Expression of miR-377-3p and CCNG2 mRNA in plasma and placenta of pregnant women with PE was correlated with systolic, diastolic blood pressure and neonatal birth weight. miR-377-3p and CCNG2 were consistently expressed in plasma and placental tissues, and their expressions were negatively correlated. Dual luciferase assay showed that miR-377-3p negatively regulated CCNG2 expression in HTR-8/SVneo cells. Conclusion Aberrant expression of miR-377-3p and CCNG2 in pregnant women with PE was associated with the severity of PE and its prognosis. It is hypothesized that miR-377-3p may be involved in the pathogenesis of PE through targeted inhibition of CCNG2 expression.