Study of some microRNA on chromosome 19 (C19MC) in serum and breast cancer tissue

Author:

Altalebi Saif Adil Ridha1,Haghi Mehdi1ORCID,Feizi Mohammad Ali Hosseinpour1

Affiliation:

1. University of Tabriz

Abstract

Abstract Background Breast cancer (BC) is the most prevalent cancer among females worldwide. Many studies suggest that certain RNAs play a role in carcinogenesis. The primate-specific microRNA gene cluster on chromosome 19 q27.3 region (C19MC) could regulate tumor cell proliferation, migration, and invasion. Objective In this study, we compared the expression of miRNAs of the C19MC cluster in breast cancer tumor and non-tumor samples, as well as in the serum of BC-affected and healthy individuals. Methods Peripheral blood was collected from 100 people with BC and 100 healthy individuals, and breast cancer samples of tumor and margin tissue were collected. After RNA extraction, cDNA was synthesized using RT-PCR. The expression of C19MC, including miR-515-1, miR-515-2, miR-516-A1, miR-516-A2, miR-516-B1, miR-516-B2, miR-517-A, miR-517-B, miR-517-C, and miR-518-A1, in the control-patient serum and tissue of BC and tumor margin were investigated using real-time PCR. Statistical analyses and ROC curves were generated using GraphPad Prism software (v8.04), and a p-value of 0.05 was considered significant. Results Our findings show that high expression of all C19MC miRNAs mentioned, except miR-517-B and miR-517-C, tightly correlates with BC and can be utilized as noticeable non-invasive tumor markers. Conclusion Our data support a general effect of C19MC miRNAs on BC detection and highlight the potential role of several C19MC members in this process.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3