Clinical and Biological Significances of LINC01940 in Gastric Cancer

Abstract

Abstract Background: In recent years, long-chain non-coding RNA has attracted wide attention. In addition to playing a significant role in the genesis of cancer, it is also involved in numerous biological functions. This article aims to explore the influence of LIINC01940 on the prognosis of gastric cancer and provide new insights into it. Methods: The expression of LINC01940 in STAD and its relationship to patients' prognoses for gastric cancer were investigated using the TCGA database. The link between LINC01940 expression and STAD patient stage was investigated. GEO, KEGG, and GSEA were used to analyze their functional differences and related enrichment pathways. The immunological correlates score was determined using immunoinfiltration analysis. Furthermore assessed was the connection between STAD patients' LINC01940 expression and medication susceptibility. And verified by in vitro experiments. Results: LINC01940 was highly expressed in STAD, and the same results were obtained by PCR. Survival analysis and Kaplan-Meier curves found that the STAD patients with high levels of LINC01940 had a poor prognosis. And LINC01940 was an independent risk factor for the poor prognosis of STAD. Furthermore, the expression of LINC01940 is correlated with T-stage. KEGG showed that LINC01940 was related to processes of metabolic biology and GSEA was enriched for calcium signaling pathway, etc. There are strong associations between LINC01940 and the tumor immune microenvironment, as well as a negative relationship between it and the sensitivity of various anticancer drugs. LINC01940 inhibited gastric cancer cell proliferation, migration, and invasion. Conclusion: LINC01940 is overexpressed in STAD, and it is a crucial element in the progression and development of gastric cancer.